Eukaryotic expression, purification, identification, and tissue distribution of porcine PID1

Expression and purification of porcine PID1 gene in Escherichia coli

* Correspondence: [email protected]

Prokaryotic expression, purification, polyclonal antibody preparation, and tissue distribution of porcine Six1

Cloning and Expression of VP2 Gene of Infectious Bursal Disease Virus in Eukaryotic Cells

Infectious bursal disease (IBD) is an economically important viral disease of chickens with worldwide distribution which suppresses the immune system of young chickens. VP2 is the major host-protective protein of infectious bursal disease virus (IBDV). The encoding region of VP2 protein was PCR amplified from a plasmid containing a cDNA fragment of large genomic segment of IBDV, strain D78. Thi...

P-130: Designing and Construction of An Appropriate Eukaryotic Expression Vector to Generate Soluble Form of Human Hyaluronidase Type PH20 in Cell Culture Feasible for Application in IVF and ICSI

Background: The hyaluronidases are the enzymes hydrolyze β-1, 4 glycosidic linkage of hyaluronan. Hyaluronan is a polymer consisting of a repeating disaccharide unit found in cumulus ovuforus complex, semen liquid and other tissue. Addition to hydrolyzing the hyaluronan, hyaluronidase can penetrate through the cumulus cells layer that surrounds the oocyte, thus it terms spreading factor. Moreov...

Design and Cloning of the Optimized L1 Gene from Human Papilloma virus 18 into the Expression Vector PcDNA3 and Evaluating its Expression in a Eukaryotic System

Background: Vaccines have played a special role in controlling and reducing mortality from infectious diseases. In this regard, DNA vaccines were developed to ease the production and reduce the risks of traditional vaccines. Human papillomavirus (HPV) has been introduced as the causing agent of cervical cancer. The capsid protein (L1) of HPV has been used to produce subunit and DNA vaccines. Th...